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CBL antibody

Anti-CBL antibody used in IHC (Paraffin sections) (IHC-P). GTX50669
Anti-CBL antibody used in Western Blot (WB). GTX50669

Cat No. GTX50669

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 698~702 (T-E-Y-M-T) derived from human CBL.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55

Cellular Localization

Cytoplasm,Cell membrane,Golgi apparatus

Background

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-CBL antibody used in IHC (Paraffin sections) (IHC-P). GTX50669

GTX50669 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50669 CBL antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-CBL antibody used in Western Blot (WB). GTX50669

GTX50669 WB Image

WB analysis of extracts from HeLa and HT-29 cells using GTX50669 CBL antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf