APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 674 (A-I-Y(p)-S-L) derived from human CBL.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55
Cellular Localization
Cytoplasm,Cell membrane,Golgi apparatus
Background
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
|
GTX55390 WB Image
WB analysis of extracts from HepG2 cells treated with Na2VO3 using GTX55390 CBL (phospho Tyr674) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
|
REFERENCE
There are currently no references for CBL (phospho Tyr674) antibody (GTX55390). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CBL (phospho Tyr674) antibody (GTX55390). Be the first to share your experience with this product.
![Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624](/upload/website/prouct_img/small/GTX108624/GTX108624_43754_20191108_WB_KO_watermark_20092220_924.jpg "Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624")
