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CBL (phospho Tyr700) antibody

Anti-CBL (phospho Tyr700) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55004
Anti-CBL (phospho Tyr700) antibody used in IHC (Paraffin sections) (IHC-P). GTX55004
Anti-CBL (phospho Tyr700) antibody used in Western Blot (WB). GTX55004

Cat No. GTX55004

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of CBL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cbl proto-oncogene , C-CBL , CBL2 , FRA11B , NSLL , RNF55

Cellular Localization

Cytoplasm,Cell membrane,Golgi apparatus

Background

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-CBL (phospho Tyr700) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55004

GTX55004 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55004 CBL (phospho Tyr700) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-CBL (phospho Tyr700) antibody used in IHC (Paraffin sections) (IHC-P). GTX55004

GTX55004 IHC-P Image

IHC-P analysis of formalin fixed human lymph node tissue section using GTX55004 CBL (phospho Tyr700) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-CBL (phospho Tyr700) antibody used in Western Blot (WB). GTX55004

GTX55004 WB Image

WB analysis of UV-treated HT29 (A), NIH3T3 (B), rat kidney (C) whole cell lysates using GTX55004 CBL (phospho Tyr700) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339