GeneTex
United States (US)

CBP (acetyl Lys1535) antibody

CBP-acetyl-Lys1535-antibody-GTX86958-IHC-1_18121409_631.jpg

Cat No. GTX86958

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

IHC: 1:50~1:100
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

265 kDa. ( Note )

Specificity/Sensitivity

CBP (Acetyl-Lys1535) Antibody detects endogenous levels of total CBP protein only when acetylated at lysine1535.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CBP around the acetylated site of Lys1535.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Creb Binding Protein,Cbp,Kat3A,Rsts,Rsts1,Crebbp

Cellular Localization

Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.

Background

Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.

Database

Research Area

DATA IMAGES
CBP-acetyl-Lys1535-antibody-GTX86958-IHC-1_18121409_631.jpg

GTX86958 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CBP (Acetyl-Lys1535) Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339