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CBP antibody [C3], C-term

KO/KD
GTX101249_39721_IP_19011506_113.jpg
GTX101249_39721_20170525_WB_KO_watermark_19011506_645.jpg
GTX101249_42844_20170712_IFA_19011506_165.jpg
GTX101249_39721_IHC_M_3_19011506_638.jpg
GTX101249_39721_IHC_19011506_720.jpg
GTX101249_42844_20170525_WB_19011506_316.jpg
GTX101249_39721_IHC_M_2_19011506_159.jpg
GTX101249_42844_20170525_WB_2_19011506_578.jpg
GTX101249_39721_IHC_M_19011506_562.jpg
GTX101249_39721_ChIP_19011506_261.jpg
GTX101249_39721_IHC_2_19011506_213.jpg

Cat No. GTX101249

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT-116 , *Vero E6

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CBP antibody, KAT3A antibody, RSTS antibody, CREBBP antibody, CREB-binding protein antibody, CREB binding protein antibody

Cellular Localization

Nucleus

Background

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101249_39721_IP_19011506_113.jpg

GTX101249 IP Image

CBP antibody immunoprecipitates CBP protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CBP protein by 2.5 μg of CBP antibody (GTX101249) 12% SDS-PAGE The immunoprecipitated CBP protein was detected by CBP antibody (GTX101249) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101249_39721_20170525_WB_KO_watermark_19011506_645.jpg

GTX101249 WB Image

KO/KD

Wild-type (WT) and CBP knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101249_42844_20170712_IFA_19011506_165.jpg

GTX101249 ICC/IF Image

CBP antibody [C3], C-term detects CBP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CBP protein stained by CBP antibody [C3], C-term (GTX101249) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

GTX101249_39721_IHC_M_3_19011506_638.jpg

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249_39721_IHC_19011506_720.jpg

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249_42844_20170525_WB_19011506_316.jpg

GTX101249 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101249_39721_IHC_M_2_19011506_159.jpg

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249_42844_20170525_WB_2_19011506_578.jpg

GTX101249 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101249_39721_IHC_M_19011506_562.jpg

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249_39721_ChIP_19011506_261.jpg

GTX101249 ChIP assay Image

CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of CREBBP antibody (GTX101249) The precipitated DNA was detected by PCR with primer set targeting to snail promoter.

GTX101249_39721_IHC_2_19011506_213.jpg

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

CBP antibody [C3], C-term Cat No. GTX101249

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Feb-2013
Score :
Application Tested : ICC/IF
Sample Species : Mk
Sample : Vero E6 cells
Blocking : 3% BSA, 23°C, 1Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : Acetone/DPBS
Primary Antibody : 1:200, 23°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169