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CBP antibody [HL2197]

Recombinant antibody KO/KD Orthogonal
Anti-CBP antibody [HL2197] used in Western Blot (WB). GTX638201
Anti-CBP antibody [HL2197] used in Western Blot (WB). GTX638201
Anti-CBP antibody [HL2197] used in Western Blot (WB). GTX638201
Anti-CBP antibody [HL2197] used in IHC (Paraffin sections) (IHC-P). GTX638201
Anti-CBP antibody [HL2197] used in IHC (Paraffin sections) (IHC-P). GTX638201
Anti-CBP antibody [HL2197] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638201

Cat. No. GTX638201

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2197

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human CBP

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CREB binding protein , CBP , KAT3A , MKHK1 , RSTS , RSTS1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-CBP antibody [HL2197] used in Western Blot (WB). GTX638201

GTX638201 WB Image

KO/KD

Wild-type (WT) and CREBBP knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (GTX638201) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBP antibody [HL2197] used in Western Blot (WB). GTX638201

GTX638201 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (GTX638201) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CBP antibody [HL2197] used in Western Blot (WB). GTX638201

GTX638201 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (GTX638201) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CBP antibody [HL2197] used in IHC (Paraffin sections) (IHC-P). GTX638201

GTX638201 IHC-P Image

CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CBP stained by CBP antibody [HL2197] (GTX638201) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBP antibody [HL2197] used in IHC (Paraffin sections) (IHC-P). GTX638201

GTX638201 IHC-P Image

CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
CBP stained by CBP antibody [HL2197] (GTX638201) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBP antibody [HL2197] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638201

GTX638201 ICC/IF Image

CBP antibody [HL2197] detects CBP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CBP stained by CBP antibody [HL2197] (GTX638201) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations