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CBP antibody

KO/KD
Anti-CBP antibody used in Western Blot (WB). GTX56255
Anti-CBP antibody used in Western Blot (WB). GTX56255
Anti-CBP antibody used in Western Blot (WB). GTX56255
Anti-CBP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56255
Anti-CBP antibody used in IHC (Paraffin sections) (IHC-P). GTX56255

Cat. No. GTX56255

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all CBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of CBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CREB binding protein , CBP , KAT3A , MKHK1 , RSTS , RSTS1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-CBP antibody used in Western Blot (WB). GTX56255

GTX56255 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody (GTX56255) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CBP antibody used in Western Blot (WB). GTX56255

GTX56255 WB Image

KO/KD

Wild-type (WT) and CBP knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody (GTX56255) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBP antibody used in Western Blot (WB). GTX56255

GTX56255 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody (GTX56255) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56255

GTX56255 ICC/IF Image

ICC/IF analysis of formalin-fixed A431 cells using GTX56255 CBP antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-CBP antibody used in IHC (Paraffin sections) (IHC-P). GTX56255

GTX56255 IHC-P Image

IHC-P analysis of formalin fixed human tonsil tissue section using GTX56255 CBP antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399