GeneTex
United States (US)

CBP80 antibody [N2], N-term

GTX106328_IFA_19011506_284.jpg
GTX106328_WB_19011506_438.jpg
GTX106328_39946_IHC_19011506_805.jpg

Cat No. GTX106328

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CBP80. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CBP80 antibody, MGC2087 antibody, NCBP antibody, Sto1 antibody, NCBP1 antibody, 80 kDa nuclear cap-binding protein antibody, nuclear cap-binding protein subunit 1 antibody, NCBP 80 kDa subunit antibody, "nuclear cap binding protein subunit 1, 80kDa antibody"

Cellular Localization

Nucleus

Background

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq]

Database

DATA IMAGES
GTX106328_IFA_19011506_284.jpg

GTX106328 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP80 (GTX106328) antibody at 1:200 dilution.

GTX106328_WB_19011506_438.jpg

GTX106328 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106328 diluted at 1:2000

GTX106328_39946_IHC_19011506_805.jpg

GTX106328 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using NCBP1(GTX106328) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169