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CBS antibody

Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Immunoprecipitation (IP). GTX113400
Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400

Cat No. GTX113400

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse liver , 293T , HeLa , CBS-transfected 293T

Predict Reactivity

Rabbit, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036461

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cystathionine-beta-synthase , CBSL , HIP4

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400

GTX113400 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS(GTX113400) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

CBS antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113400

GTX113400 ICC/IF Image

CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CBS stained by CBS antibody (GTX113400) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Immunoprecipitation (IP). GTX113400

GTX113400 IP Image

CBS antibody immunoprecipitates CBS protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CBS protein by 4 μg CBS antibody (GTX113400)
7.5 % SDS-PAGE
The immunoprecipitated CBS protein was detected by CBS antibody (GTX113400) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400

GTX113400 IHC-P Image

CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CBS stained by CBS antibody (GTX113400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169