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CBX1 / HP1 beta antibody

Anti-CBX1 / HP1 beta antibody used in Western Blot (WB). GTX106418
Anti-CBX1 / HP1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106418
Anti-CBX1 / HP1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106418
Anti-CBX1 / HP1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106418
Anti-CBX1 / HP1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106418

Cat. No. GTX106418

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CBX1 / HP1 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CBX1 / HP1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949863

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 1 , CBX , HP1-BETA , HP1Hs-beta , HP1Hsbeta , M31 , MOD1 , p25beta

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CBX1 / HP1 beta antibody used in Western Blot (WB). GTX106418

GTX106418 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX106418 diluted at 1:1000
Anti-CBX1 / HP1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106418

GTX106418 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
CBX1 / HP1 beta antibody (GTX106418) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBX1 / HP1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106418

GTX106418 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HP1-beta(GTX106418) antibody at 1:200 dilution.

Anti-CBX1 / HP1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106418

GTX106418 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 protein at nucleus on C2C12 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded C2C12 xenograft.
CBX1 / HP1 beta antibody (GTX106418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBX1 / HP1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX106418

GTX106418 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
CBX1 / HP1 beta antibody (GTX106418) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Acquaviva L et al. Nature 2020; 582 (7812) : 426-431 Ensuring meiotic DNA break formation in the mouse pseudoautosomal region.
Application : ICC/IF
Reactivity : Mouse
Li Y et al. Nucleic Acids Res 2018; (Epub) Histone H1 acetylation at lysine 85 regulates chromatin condensation and genome stability upon DNA damage.
Application : WB
Reactivity : Human
Scherthan H et al. Chromosoma 2014; Contrasting behavior of heterochromatic and euchromatic chromosome portions and pericentric genome separation in pre-bouquet spermatocytes of hybrid mice.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169