GeneTex
United States (US)

CBX1 / HP1 beta antibody

GTX106418_39890_20150116_IHC_M_19011506_499.jpg
GTX106418_IFA_19011506_106.jpg
GTX106418_39890_IHC_19011506_847.jpg
GTX106418_39890_20150116_IHC_M_2_19011506_612.jpg
GTX106418_WB_19011506_361.jpg

Cat No. GTX106418

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CBX1 / HP1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CBX antibody, HP1-BETA antibody, HP1Hs-beta antibody, HP1Hsbeta antibody, M31 antibody, MOD1 antibody, p25beta antibody, CBX1 antibody, chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody, chromobox protein homolog 1 antibody, heterochromatin protein 1-beta antibody, heterochromatin protein 1 homolog beta antibody, heterochromatin protein p25 beta antibody, modifier 1 protein antibody, HP1 beta homolog antibody, chromobox homolog 1 antibody

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
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Database

DATA IMAGES
GTX106418_39890_20150116_IHC_M_19011506_499.jpg

GTX106418 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
CBX1 / HP1 beta antibody (GTX106418) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106418_IFA_19011506_106.jpg

GTX106418 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HP1-beta(GTX106418) antibody at 1:200 dilution.

GTX106418_39890_IHC_19011506_847.jpg

GTX106418 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 protein at nucleus on C2C12 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded C2C12 xenograft.
CBX1 / HP1 beta antibody (GTX106418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106418_39890_20150116_IHC_M_2_19011506_612.jpg

GTX106418 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
CBX1 / HP1 beta antibody (GTX106418) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106418_WB_19011506_361.jpg

GTX106418 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX106418 diluted at 1:1000

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169