APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CBX1 / HP1 beta. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10728930
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chromobox 1 , CBX , HP1-BETA , HP1Hs-beta , HP1Hsbeta , M31 , MOD1 , p25beta
Cellular Localization
Nucleus
Background
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115510 IHC-P Image
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using CBX1 / HP1 beta(GTX115510) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115510 IHC-P Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CBX1 / HP1 beta(GTX115510) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115510 IHC-P Image
CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CBX1 / HP1 beta antibody (GTX115510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX115510 WB Image
Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115510 diluted at 1:1000
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REFERENCE
There are currently no references for CBX1 / HP1 beta antibody (GTX115510). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CBX1 / HP1 beta antibody (GTX115510). Be the first to share your experience with this product.
