GeneTex
United States (US)

CBX1 / HP1 beta antibody

GTX115510_40394_20150814_IHC_M_18121410_925.jpg
GTX115510_40394_WB_18121410_347.jpg
GTX115510_40394_IHC_18121410_274.jpg
GTX115510_40394_IHC_M_18121410_895.jpg

Cat No. GTX115510

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HeLa , HepG2 , HCT116

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBX1 / HP1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chromobox 1,Cbx,Hp1-Beta,Hp1Hs-Beta,Hp1Hsbeta,M31,Mod1,P25Beta,Cbx1

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX115510_40394_20150814_IHC_M_18121410_925.jpg

GTX115510 IHC-P Image

CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CBX1 / HP1 beta antibody (GTX115510) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115510_40394_WB_18121410_347.jpg

GTX115510 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115510 diluted at 1:1000

GTX115510_40394_IHC_18121410_274.jpg

GTX115510 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using CBX1 / HP1 beta(GTX115510) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115510_40394_IHC_M_18121410_895.jpg

GTX115510 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CBX1 / HP1 beta(GTX115510) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169