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CBX4 antibody

Anti-CBX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24189
Anti-CBX4 antibody used in Western Blot (WB). GTX24189
Anti-CBX4 antibody used in Western Blot (WB). GTX24189

Cat. No. GTX24189

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
100 μg ($589)
See all CBX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:4000
ICC/IF 1:100-1:400
IP 1:100
ELISA 1:10000-1:40000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.

Purification

Purified by antigen-affinity chromatography.
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 4 , NBP16 , PC2

Cellular Localization

Nucleus,Nucleus speckle

Background

PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.

Database

Research Area

DATA IMAGES

Anti-CBX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24189

GTX24189 ICC/IF Image

GeneTex anti-hPC2 antibody (GTX24189) was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37º C. Nuclei were counterstained with Hoechst stain (blue).

Anti-CBX4 antibody used in Western Blot (WB). GTX24189

GTX24189 WB Image

Western blotting. Analysis shows the detection of human PC2 in probed lysates using GeneTex Affinity Purified anti-hPC2 antibody (GTX24189). The panel on the left shows the blot probed with anti-hPC2. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2. In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells.

Anti-CBX4 antibody used in Western Blot (WB). GTX24189

GTX24189 WB Image

WB analysis of flag-tagged human CBX4 protein using GTX24189 CBX4 antibody. Panel on the left shows the blot probed with GTX24189 CBX4 antibody. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant CBX4 in the lysate.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589