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CCDC14 antibody [C2C3], C-term

Anti-CCDC14 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120754
Anti-CCDC14 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120754
Anti-CCDC14 antibody [C2C3], C-term used in Western Blot (WB). GTX120754
Anti-CCDC14 antibody [C2C3], C-term used in Western Blot (WB). GTX120754

Cat No. GTX120754

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

293T , A431 , U87-MG , SK-N-SH , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CCDC14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coiled-coil domain containing 14

Database

DATA IMAGES

Anti-CCDC14 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120754

GTX120754 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CCDC14(GTX120754) antibody at 1:500 dilution.

Anti-CCDC14 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120754

GTX120754 ICC/IF Image

CCDC14 antibody [C2C3], C-term detects CCDC14 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CCDC14 protein stained by CCDC14 antibody [C2C3], C-term (GTX120754) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-CCDC14 antibody [C2C3], C-term used in Western Blot (WB). GTX120754

GTX120754 WB Image

Sample (30 μg of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX120754 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCDC14 antibody [C2C3], C-term used in Western Blot (WB). GTX120754

GTX120754 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCDC14 antibody [C2C3], C-term (GTX120754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

CCDC14 antibody [C2C3], C-term Cat No. GTX120754

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26297806
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26297806
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26297806
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26297806
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26297806
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169