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CCDC68 antibody [N2C3]

KO/KD Orthogonal
Anti-CCDC68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106883
Anti-CCDC68 antibody [N2C3] used in Western Blot (WB). GTX106883
Anti-CCDC68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106883
Anti-CCDC68 antibody [N2C3] used in Western Blot and Immunoprecipitation (WB IP). GTX106883
Anti-CCDC68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106883

Cat. No. GTX106883

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CCDC68 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Raji

Product Note

Knockdown/Knockout validation was supported by references (PMID:28422092).

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CCDC68. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949871

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coiled-coil domain containing 68 , SE57-1

Database

DATA IMAGES

Anti-CCDC68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106883

GTX106883 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CCDC68(GTX106883) antibody at 1:200 dilution.

Anti-CCDC68 antibody [N2C3] used in Western Blot (WB). GTX106883

GTX106883 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX106883 diluted at 1:1000

Anti-CCDC68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106883

GTX106883 ICC/IF Image

Orthogonal

The data was published in the journal Nat Commun in 2017.PMID: 28422092

Anti-CCDC68 antibody [N2C3] used in Western Blot and Immunoprecipitation (WB IP). GTX106883

GTX106883 WB IP Image

The data was published in the journal Nat Commun in 2017.PMID: 28422092

Anti-CCDC68 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106883

GTX106883 ICC/IF Image

The data was published in the journal Nat Commun in 2017.PMID: 28422092

REFERENCE

Application Reference

Li Q et al. Adv Sci (Weinh) 2024; 11 (35) : e2406009 CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC.
Ning Huang et al. Nat Commun 2017; 8 : 15057 Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169