GeneTex
United States (US)

CCKBR antibody [C1C2], Internal

GTX100347_39568_IHC_19011506_709.jpg
GTX100347_39568_20170518_WB_19011506_902.jpg
GTX100347_39568_IHC_2_19011506_669.jpg

Cat No. GTX100347

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Unboiled HeLa cell extract

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CCKBR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cholecystokinin B Receptor,Cck-B,Cck2R,Gasr,Cckbr

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100347_39568_IHC_19011506_709.jpg

GTX100347 IHC-P Image

CCKBR antibody [C1C2], Internal detects CCKBR protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma .
CCKBR antibody [C1C2], Internal (GTX100347) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100347_39568_20170518_WB_19011506_902.jpg

GTX100347 WB Image

Unboiled HeLa cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCKBR antibody (GTX100347) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100347_39568_IHC_2_19011506_669.jpg

GTX100347 IHC-P Image

CCKBR antibody [C1C2], Internal detects CCKBR protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: CCKBR antibody [C1C2], Internal (GTX100347) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169