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CCKBR antibody [C1C2], Internal

Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347
Anti-CCKBR antibody [C1C2], Internal used in Western Blot (WB). GTX100347
Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347

Cat No. GTX100347

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Unboiled HeLa cell extract
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CCKBR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cholecystokinin B receptor,CCK-B,CCK2R,GASR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347

GTX100347 IHC-P Image

CCKBR antibody [C1C2], Internal detects CCKBR protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma .
CCKBR antibody [C1C2], Internal (GTX100347) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCKBR antibody [C1C2], Internal used in Western Blot (WB). GTX100347

GTX100347 WB Image

Unboiled HeLa cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCKBR antibody (GTX100347) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347

GTX100347 IHC-P Image

CCKBR antibody [C1C2], Internal detects CCKBR protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: CCKBR antibody [C1C2], Internal (GTX100347) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for CCKBR antibody [C1C2], Internal (GTX100347). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CCKBR antibody [C1C2], Internal (GTX100347). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169