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CCKBR antibody [C1C2], Internal

Anti-CCKBR antibody [C1C2], Internal used in Western Blot (WB). GTX100347
Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347
Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347

Cat. No. GTX100347

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CCKBR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Unboiled HeLa cell extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CCKBR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240565

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholecystokinin B receptor , CCK-B , CCK2R , GASR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCKBR antibody [C1C2], Internal used in Western Blot (WB). GTX100347

GTX100347 WB Image

Unboiled HeLa cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCKBR antibody (GTX100347) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347

GTX100347 IHC-P Image

CCKBR antibody [C1C2], Internal detects CCKBR protein at membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma .
CCKBR antibody [C1C2], Internal (GTX100347) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCKBR antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100347

GTX100347 IHC-P Image

CCKBR antibody [C1C2], Internal detects CCKBR protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: CCKBR antibody [C1C2], Internal (GTX100347) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169