GeneTex
United States (US)

CCL14 antibody

GTX108428_WB_19011507_892.jpg
GTX108428_39848_IHC_19011507_886.jpg

Cat No. GTX108428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

CCL14 tansfected 293T cell
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CCL14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C Motif Chemokine Ligand 14,Cc-1,Cc-3,Ckb1,Hcc-1,Hcc-1(1-74),Hcc-1/Hcc-3,Hcc-3,Mcif,Ncc-2,Ncc2,Scya14,Scyl2,Sy14,Ccl14

Cellular Localization

Secreted

Background

This gene, CCL14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts; it is unknown if its monocistronic transcript is also subject to alternative splicing. [provided by RefSeq]

Database

DATA IMAGES
GTX108428_WB_19011507_892.jpg

GTX108428 WB Image

Western Blot analysis of CCL14 expression in transfected 293T cell line by CCL14 polyclonal antibody.
A: Non-transfected lysate.
B: CCL14 transfected lysate.
15% SDS PAGE
GTX108428 diluted at 1:500

GTX108428_39848_IHC_19011507_886.jpg

GTX108428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CCL14(GTX108428) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169