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CCL14 antibody

Anti-CCL14 antibody used in IHC (Paraffin sections) (IHC-P). GTX108428
Anti-CCL14 antibody used in Western Blot (WB). GTX108428

Cat. No. GTX108428

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CCL14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

CCL14 transfected 293T cell

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CCL14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949878

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine ligand 14 , CC-1 , CC-3 , CKB1 , HCC-1 , HCC-1(1-74) , HCC-1/HCC-3 , HCC-3 , MCIF , NCC-2 , NCC2 , SCYA14 , SCYL2 , SY14

Cellular Localization

Secreted

Background

This gene, CCL14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts; it is unknown if its monocistronic transcript is also subject to alternative splicing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCL14 antibody used in IHC (Paraffin sections) (IHC-P). GTX108428

GTX108428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CCL14(GTX108428) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCL14 antibody used in Western Blot (WB). GTX108428

GTX108428 WB Image

Western Blot analysis of CCL14 expression in transfected 293T cell line by CCL14 polyclonal antibody.
A: Non-transfected lysate.
B: CCL14 transfected lysate.
15% SDS PAGE
GTX108428 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169