GeneTex
United States (US)

CCL21 antibody

Cat No. GTX10364

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, Neut
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/mL
Neut Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Specificity/Sensitivity

The antibody has the ability to neutralize the biological activity of recombinant mouse 6Ckine. It exhibits less than 15% cross-reactivity with recombinant human 6Ckine.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

recombinant mouse 6Ckine, expressed in Escherichia coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

O00585, MGC34555, TCA4, ECL, SCYA21, CCL21, 6Ckine, 602737, SLC, 6366, CKb9

Cellular Localization

Secreted

Background

Mouse 6Ckine is a beta- or C-C chemokine identified in the Expressed Sequence Tag (EST) database by three independent groups (Hedrick and Zlotnik, Hromas et al, Nagira et al). Known also as Exodus-2 and secondary lymphoid-tissue chemokine (SLC), 6Ckine contains four conserved cysteine (C) residues which are characteristic of beta-chemokines (Hedrick and Zlotnik, Hromas et al, Nagira et al). Two additional conserved cysteine residues have been found in its unusually long carboxy-terminal domain and consequently the name 6Ckine (Hedrick and Zlotnik, Hromas et al, Nagira et al). Human and mouse 6Ckine are highly conserved and show 86% amino acid homology.1 Mouse 6Ckine cDNA encodes a 133 amino acid precursor protein, a 23 amino acid signal peptide and a 110 amino acid mature protein. (Hedrick and Zlotnik). Comparatively, human 6Ckine cDNA encodes a 134 amino acid precursor protein, a 23 amino acid signal peptide and a 111 amino acid mature protein(Hedrick and Zlotnik, Nagira et al). Recombinant mouse 6Ckine has a predicted molecular mass of approximately 12 kDa (mature protein). In SDS-PAGE under reducing and non-reducing conditions, the recombinant protein migrates with an apparent molecular mass of 16 kDa to 17 kDA.The expression of mouse 6Ckine has been detected in high levels in the spleen and lung (Nagira et al). Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells (Hedrick and Zlotnik). Unlike other C-C chemokines, 6Ckine is not chemotactic for monocytes and neutrophils (Hedrick and Zlotnik, Nagira et al). A growing body of work suggests that 6Ckine influences lymphocyte homing to secondary lymphoid organs (Gunn et al), integrin-mediated lymphocyte adhesion (Pachynski et al) and may act via the EBI1 ligand chemokine (ELC) receptor, CCR7 (Willimann et al, Yoshida et al).

Database

SDS
PBS.pdf
Package List Price ($)
$ 429