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CCN3 antibody

Anti-CCN3 antibody used in Western Blot (WB). GTX103377
Anti-CCN3 antibody used in Western Blot (WB). GTX103377
Anti-CCN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103377
Anti-CCN3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103377
Anti-CCN3 antibody used in Western Blot (WB). GTX103377

Cat No. GTX103377

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , mouse brain , human CCN3-transfected 293T cells

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CCN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951010

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cellular communication network factor 3 , IBP-9 , IGFBP-9 , IGFBP9 , NOV , NOVh

Cellular Localization

Secreted

Background

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody (GTX103377) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Sample(30 μg whole cell lysate)
A:293T
12% SDS PAGE
GTX103377 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103377

GTX103377 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CCN3 (GTX103377) antibody at 1:200 dilution.

Anti-CCN3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103377

GTX103377 IHC-P Image

Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using NOV(GTX103377) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103377 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

CCN3 antibody Cat No. GTX103377

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : mice ventricle
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : ISO: Isoproterenol
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169