GeneTex
United States (US)

CCN3 antibody

Overexpression
GTX103377_39974_20160204_WB_B_19011506_573.jpg
GTX103377_WB_19011506_215.jpg
GTX103377_IFA_19011506_616.jpg
GTX103377_IHC_19011506_797.jpg
GTX103377_39974_WB_M_brain_19011506_699.jpg

Cat No. GTX103377

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , mouse brain , human CCN3-transfected 293T cells

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CCN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCN3 antibody, IGFBP9 antibody, NOV antibody, nephroblastoma-overexpressed gene protein homolog antibody, IBP-9 antibody, IGF-binding protein 9 antibody, protein NOV homolog antibody, insulin-like growth factor-binding protein 9 antibody, novH antibody, CCN family member 3 antibody, IGFBP-9 antibody, nephroblastoma overexpressed gene antibody

Cellular Localization

Secreted

Background

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103377_39974_20160204_WB_B_19011506_573.jpg

GTX103377 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody (GTX103377) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103377_WB_19011506_215.jpg

GTX103377 WB Image

Sample(30 µg whole cell lysate)
A:293T
12% SDS PAGE
GTX103377 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103377_IFA_19011506_616.jpg

GTX103377 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CCN3 (GTX103377) antibody at 1:200 dilution.

GTX103377_IHC_19011506_797.jpg

GTX103377 IHC-P Image

Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using NOV(GTX103377) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103377_39974_WB_M_brain_19011506_699.jpg

GTX103377 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103377 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

CCN3 antibody Cat No. GTX103377

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : mice ventricle
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, overnightHr
Figure Note : ISO: Isoproterenol
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169