GeneTex
United States (US)

CCNDBP1 antibody [C2C3], C-term

GTX106440_39715_IHC_19011506_314.jpg
GTX106440_WB_19011506_442.jpg

Cat No. GTX106440

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Cyclin-D1-binding protein 1 tansfected 293T cell

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CCNDBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DIP1 antibody, GCIP antibody, CCNDBP1 antibody, grap2 and cyclin-D-interacting protein antibody, cyclin-D1-binding protein 1 antibody, human homolog of Maid antibody, cyclin D-type binding-protein 1 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Two alternatively spliced variants, which encode distinct isoforms, have been reported. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106440_39715_IHC_19011506_314.jpg

GTX106440 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitrial, using CCNDBP1(GTX106440) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106440_WB_19011506_442.jpg

GTX106440 WB Image

Western Blot analysis of CCNDBP1 expression in transfected 293T cell line by CCNDBP1 polyclonal antibody.
A: Non-transfected lysate.
B: CCNDBP1 transfected lysate.
12% SDS PAGE
GTX106440 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf