CCR1 antibody [HL2840]

Non-transfected (–) and transfected (+) unboiled K562 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL2840] (GTX640111) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL2840] (GTX640111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

CCR1 antibody [HL2840] detects CCR1 protein by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CCR1 stained by CCR1 antibody [HL2840] (GTX640111) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

CCR1 antibody [HL2840] detects CCR1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
CCR1 stained by CCR1 antibody [HL2840] (GTX640111) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA expression data for the same tissues are based on NCBI.

Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL2840] (GTX640111) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR1 antibody [HL2840] (GTX640111) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CCR1 antibody [HL2840] detects CCR1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CCR1 stained by CCR1 antibody [HL2840] (GTX640111) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min