APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/1,000 |
| 20 μg/ml |
|
Note :
IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes the N-terminal region of the chemokine receptor CCR5.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide corresponding to amino acids 6 to 20 of human CCR5.
Purification
Purified IgG
Conjugation
Unconjugated
RRID
AB_376254
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-C motif chemokine receptor 5 (gene/pseudogene) , CC-CKR-5 , CCCKR5 , CCR-5 , CD195 , CKR-5 , CKR5 , CMKBR5 , IDDM22
Cellular Localization
Cell membrane
Background
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015]
Database
Research Area
DATA IMAGES
|
GTX74371 IHC-P Image
IHC-P analysis of human lymph node tissue using GTX74371 CCR5 antibody.
|
|
|
GTX74371 WB Image
WB analysis of THP-1 cell lysate using GTX74371 CCR5 antibody.
|
REFERENCE
There are currently no references for CCR5 antibody (GTX74371). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CCR5 antibody (GTX74371). Be the first to share your experience with this product.
