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CCR6 antibody [N1N2], N-term

Anti-CCR6 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109647
Anti-CCR6 antibody [N1N2], N-term used in Western Blot (WB). GTX109647
Anti-CCR6 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109647

Cat. No. GTX109647

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CCR6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human CCR6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949901

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine receptor 6 , BN-1 , C-C CKR-6 , CC-CKR-6 , CCR-6 , CD196 , CKR-L3 , CKRL3 , CMKBR6 , DCR2 , DRY6 , GPR29 , GPRCY4 , STRL22

Background

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCR6 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109647

GTX109647 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU-145 xenograft, using CCR6(GTX109647) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCR6 antibody [N1N2], N-term used in Western Blot (WB). GTX109647

GTX109647 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
B: Molt-4 (GTX27912)
C: Raji
12% SDS PAGE
GTX109647 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCR6 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109647

GTX109647 IHC-P Image

CCR6 antibody [N1N2], N-term detects CCR6 protein at membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
CCR6 antibody [N1N2], N-term (GTX109647) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kiso K et al. PLoS One 2017; 12 (9) : e0184738 Transgelin-2 is upregulated on activated B-cells and expressed in hyperplastic follicles in lupus erythematosus patients.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169