CCR7 antibody [HL3074]

CCR7 antibody [HL3074] (GTX640520) detects CCR7 protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with CCR7 antibody [HL3074] (GTX640520)diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

CCR7 antibody [HL3074] (GTX640520) detects CCR7 protein by flow cytometry analysis.
Sample: Raji were blocked with an FcR blocking reagent for 10 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with CCR7 antibody [HL3074] (GTX640520) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

CCR7 antibody [HL3074] detects CCR7 protein by immunofluorescent analysis.
Sample: MJ cells were fixed in ice-cold MeOH for 5 min.
Green: CCR7 stained by CCR7 antibody [HL3074] (GTX640520) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

CCR7 antibody [HL3074] detects CCR7 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human spleen.
CCR7 stained by CCR7 antibody [HL3074] (GTX640520) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CCR7 antibody [HL3074] detects CCR7 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: CCR7 stained by CCR7 antibody [HL3074] (GTX640520) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).