CCR9 antibody [HL3054]

CCR9 antibody [HL3054] (GTX640497) detects CCR9 protein by flow cytometry analysis.
Sample: non-fixed and non-permeabilized Molt-4 or HeLa cells were stained with 5μg/ml GTX640497 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4ºC for 1 hour. Molt-4 cells staining with FcR Blocking treatment 10 mins at 4ºC.

CCR9 antibody [HL3054] detects CCR9 protein by immunofluorescent analysis.
Sample: Molt-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CCR9 stained by CCR9 antibody [HL3054] (GTX640497) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

CCR9 antibody [HL3054] detects CCR9 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: CCR9 stained by CCR9 antibody [HL3054] (GTX640497) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR9 antibody [HL3054] (GTX640497) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

CCR9 antibody [HL3054] detects CCR9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
CCR9 stained by CCR9 antibody [HL3054] (GTX640497) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min