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CD10 antibody [3G9D10]

Anti-CD10 antibody [3G9D10] used in Western Blot (WB). GTX83175
Anti-CD10 antibody [3G9D10] used in IHC (Paraffin sections) (IHC-P). GTX83175

Cat. No. GTX83175

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G9D10

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of CD10 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane metalloendopeptidase , CALLA , CD10 , CMT2T , NEP , SCA43 , SFE

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD10 antibody [3G9D10] used in Western Blot (WB). GTX83175

GTX83175 WB Image

WB analysis of truncated CD10-His recombinant protein using GTX83175 CD10 antibody [3G9D10].

Anti-CD10 antibody [3G9D10] used in IHC (Paraffin sections) (IHC-P). GTX83175

GTX83175 IHC-P Image

IHC-P analysis of human breast ductal myoepithelium using GTX83175 CD10 antibody [3G9D10].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399