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CD10 antibody [N2C1], Internal

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680
Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680
Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680
Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680
Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680

Cat. No. GTX111680

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

rat kidney

Predict Reactivity

Rabbit, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726415

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane metalloendopeptidase , CALLA , CD10 , CMT2T , NEP , SCA43 , SFE

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680

GTX111680 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal (GTX111680) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680

GTX111680 WB Image

CD10 antibody [N2C1], Internal detects MME protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
7.5% SDS-PAGE
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD10 antibody [N2C1], Internal used in Western Blot (WB). GTX111680

GTX111680 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal (GTX111680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680

GTX111680 IHC-P Image

CD10 antibody [N2C1], Internal detects CD10 protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111680

GTX111680 IHC-P Image

CD10 antibody [N2C1], Internal detects MME protein at cytosol and membrane on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yap XL et al. Membranes (Basel) 2021; 11 (8) Detection of Prostate Cancer via IR Spectroscopic Analysis of Urinary Extracellular Vesicles: A Pilot Study.
Application : WB
Reactivity : Human
Fukui K et al. Neurosci Res 2017; (Epub) Rebamipide reduces amyloid-β 1-42 (Aβ42) production and ameliorates Aβ43-lowered cell viability in cultured SH-SY5Y human neuroblastoma cells.
Application : WB
Reactivity : Human
Wang S et al. J Biol Chem 2013; 288 (36) : 26265-74 Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169