GeneTex
United States (US)

CD10 antibody [N2C1], Internal

GTX111680_40485_WB_18121410_280.jpg
GTX111680_40485_WB_R_kidney_18121410_618.jpg
GTX111680_40485_IHC_M_18121410_323.jpg
GTX111680_40485_IHC_18121410_473.jpg
GTX111680_40485_WB_M_kidney_18121410_568.jpg

Cat No. GTX111680

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Jurkat , mouse kidney , rat kidney

Predict Reactivity

Rabbit, Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.86mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CALLA antibody, CD10 antibody, DKFZp686O16152 antibody, MGC126681 antibody, MGC126707 antibody, NEP antibody, SFE antibody, MME antibody, membrane metallo-endopeptidase variant 2 antibody, neprilysin antibody, atriopeptidase antibody, enkephalinase antibody, membrane metallo-endopeptidase variant 1 antibody, membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase) antibody, skin fibroblast elastase antibody, neutral endopeptidase 24.11 antibody, membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody, common acute lymphocytic leukemia antigen antibody, membrane metallo-endopeptidase antibody

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX111680_40485_WB_18121410_280.jpg

GTX111680 WB Image

Sample (30 µg of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX111680 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111680_40485_WB_R_kidney_18121410_618.jpg

GTX111680 WB Image

CD10 antibody [N2C1], Internal detects MME protein by western blot analysis.
A. 50 µg rat kidney lysate/extract
7.5% SDS-PAGE
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111680_40485_IHC_M_18121410_323.jpg

GTX111680 IHC-P Image

CD10 antibody [N2C1], Internal detects CD10 protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111680_40485_IHC_18121410_473.jpg

GTX111680 IHC-P Image

CD10 antibody [N2C1], Internal detects MME protein at cytosol and membrane on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111680_40485_WB_M_kidney_18121410_568.jpg

GTX111680 WB Image

CD10 antibody [N2C1], Internal detects MME protein by western blot analysis.
A. 50 µg mouse kidney lysate/extract
7.5% SDS-PAGE
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf