Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal (GTX111680) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal (GTX111680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD10 antibody [N2C1], Internal detects MME protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
7.5% SDS-PAGE
CD10 antibody [N2C1], Internal (GTX111680) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.