GeneTex
United States (US)

CD10 antibody [SN5c]

Cat No. GTX75137

Host Mouse
Clonality Monoclonal
Clone Name SN5c
Isotype IgG1
Application WB, IHC-Fr, FACS, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS 1/100-1/200
IP Assay dependent

Note :

Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

86 kDa kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Concentration

1.0mg/ml

Antigen Species

Human

Immunogen

Partially purified cell membrane antigens from fresh leukemia cells.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SFE Antibody , NEP Antibody , MME Antibody , membrane metallo-endopeptidase Antibody , CALLA Antibody

Background

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]
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Database

Research Area