GeneTex
United States (US)

CD105 antibody [N3C3]

GTX100508_39932_IHC_19011506_947.jpg
GTX100508_39932_20151119_WB_19011506_458.jpg

Cat No. GTX100508

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD105. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD105 antibody, END antibody, FLJ41744 antibody, HHT1 antibody, ORW antibody, ORW1 antibody, ENG antibody, CD105 antigen antibody, endoglin antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100508_39932_IHC_19011506_947.jpg

GTX100508 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using CD105(GTX100508) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100508_39932_20151119_WB_19011506_458.jpg

GTX100508 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 antibody [N3C3] (GTX100508) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 10625079

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169