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CD11a antibody [38] (Azide free)

Anti-CD11a antibody [38] (Azide free) used in Flow cytometry (FACS). GTX75442

Cat. No. GTX75442

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 38

Isotype

 IgG2a

Application

 ICC/IF, IHC-Fr, FACS, IP, Blocking, Neutralizing/Inhibition

Reactivity

 Human, Mouse
Package
500 μg ($509)
See all CD11a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
Blocking Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Mouse anti Human CD11a antibody, clone 38 inhibits CD11a mediated adhesion of T cells to ICAM-1 (Stewart et al. 1996).
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376805

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha L , CD11A , LFA-1 , LFA1A

Cellular Localization

Cell membrane

Background

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD11a antibody [38] (Azide free) used in Flow cytometry (FACS). GTX75442

GTX75442 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX75442 CD11a antibody [38] (Azide free).

REFERENCE

Application Reference

Hou Y et al. Ann Rheum Dis 2010; 69 (1) : 249-54 Junctional adhesion molecule-A is abnormally expressed in diffuse cutaneous systemic sclerosis skin and mediates myeloid cell adhesion.
Application : Neutralizing/Inhibition
Reactivity : Human

Clone Reference

Li L et al. Am J Transl Res 2021; 13 (10):11026-11047 Decellularized extracellular matrix loaded with IPFP-SC for repairing rabbit osteochondral defects.
Monel B et al. Cell Rep 2019; 27 (1):142-153.e4 HIV Controllers Exhibit Effective CD8+ T Cell Recognition of HIV-1-Infected Non-activated CD4+ T Cells.
Janne Lehtonen et al. Bio Protoc 2018; 8 (23):e3105 Integrin Dependent RhoB Activation Assay Using Leukocytes.
Pathak SK et al. J Biol Chem 2012; 287 (17):13731-42 Activated apoptotic cells induce dendritic cell maturation via engagement of Toll-like receptor 4 (TLR4), dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN), and β2 integrins.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 509
Custom Conjugations