GeneTex
United States (US)

CD11a antibody, Internal

GTX88390_2378_WB_18122102_366.jpg
GTX88390_984_IHC-P_18122102_560.jpg
GTX88390_983_IHC-P_18122102_453.jpg

Cat No. GTX88390

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2μg/ml
IHC-P 3.75μg/ml

Note :

IHC-P
Human Lymph Node shows membrane staining in the paracortex.

Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88390-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2; NP_001107852.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Alpha L,Cd11A,Lfa-1,Lfa1A,Itgal

Background

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88390_2378_WB_18122102_366.jpg

GTX88390 WB Image

WB analysis of human spleen lysate using GTX88390 CD11a antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

GTX88390_984_IHC-P_18122102_560.jpg

GTX88390 IHC-P Image

IHC-P analysis of human spleen using GTX88390 CD11a antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88390_983_IHC-P_18122102_453.jpg

GTX88390 IHC-P Image

IHC-P analysis of human lymph node using GTX88390 CD11a antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2µg/ml

Package List Price ($)
$ 319