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CD11b antibody [ICRF44] (FITC)

Anti-CD11b antibody [ICRF44] (FITC) used in Flow cytometry (FACS). GTX00481-06

Cat No. GTX00481-06

Host

Mouse

Clonality

Monoclonal

Clone Name

ICRF44

Isotype

IgG1

Application

FACS

Reactivity

Human, Pig, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package
100 test ($329)
PRODUCT

Summary

The ICRF44 antibody reacts with human CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 1 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Rheumatoid synovial cells and human monocytes.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit alpha M , CD11B , CR3A , MAC-1 , MAC1A , MO1A , SLEB6

Cellular Localization

Cell membrane

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD11b antibody [ICRF44] (FITC) used in Flow cytometry (FACS). GTX00481-06

GTX00481-06 FACS Image

FACS analysis of human peripheral blood monocytes using GTX00481-06 CD11b antibody [ICRF44] (FITC).
Solid line : Primary antibody
Dashed line : FITC mouse IgG1 isotype control
Antibody amount : 1 μg

REFERENCE

REVIEW

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Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329