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CD11b antibody, Internal

Anti-CD11b antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89035
Anti-CD11b antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89035

Cat. No. GTX89035

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all CD11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 10μg/ml
IHC-P Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of U937 cells.
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89035-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RNDGEDSYRTQ, from the internal region of the protein sequence according to NP_000623.2 .

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha M , CD11B , CR3A , MAC-1 , MAC1A , MO1A , SLEB6

Cellular Localization

Cell membrane

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD11b antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89035

GTX89035 ICC/IF Image

ICC/IF analysis of PFA fixed U937 cells using GTX89035 CD11b antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-CD11b antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89035

GTX89035 IHC-P Image

IHC-P analysis of human brain using GTX89035 CD11b antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399