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CD11b antibody [M1/70.15] (Low endotoxin, azide free)

Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473
Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473
Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473
Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473

Cat. No. GTX42473

Reference 5

Host

 Rat

Clonality

 Monoclonal

Clone Name

 M1/70.15

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rabbit
Package
500 μg ($739)
See all CD11b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).
Not tested in other applications.

Product Note

This antibody recognizes the murine CD11b cell surface antigen.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

T cell enriched splenocytes from B10 mice.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_11164400

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Integrin Alpha M , Cd11B/Cd18 , Cr3 , Cr3A , Cd11B , F730045J24Rik , Ly-40 , Mac1 , Mac-1 , Mac-1A , Itgam

Research Area

DATA IMAGES

Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473

GTX42473 IHC-Fr Image

IHC-Fr staining of mouse lymph node using Rat anti Mouse CD11b antibody (GTX42473), green in A and Rat anti Mouse CD8 antibody (GTX74642), red in B, Merged image in C

Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473

GTX42473 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42473 CD11b antibody [M1/70.15] (Low endotoxin, azide free).

Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473

GTX42473 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42473 CD11b antibody [M1/70.15] (Low endotoxin, azide free).

Anti-CD11b antibody [M1/70.15] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42473

GTX42473 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX42473 CD11b antibody [M1/70.15] (Low endotoxin, azide free).

REFERENCE

Application Reference

Wang HT et al. J Food Drug Anal 2018; 26 (2) : 678-687 Characteristics of fucose-containing polysaccharides from submerged fermentation of?Agaricus blazei Murill.
Zhang Z et al. Neurobiol Dis 2017; 111 : 153-162 Trajectory of inflammatory and microglial activation markers in the postnatal rabbit brain following intrauterine endotoxin exposure.
Application : IHC-P
Reactivity : Rabbit
Wang HT et al. Journal of Food and Drug Analysis 2017; (Epub) Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill.
Yang LC et al. Evid Based Complement Alternat Med 2013; 2013 : 458075 a type ii arabinogalactan from anoectochilus formosanus for g-csf production in macrophages and leukopenia improvement in ct26-bearing mice treated with 5-*.

Clone Reference

Koutarou Nakamura et al. PLoS Biol 2021; 19 (5):e3000939 Extracellular DJ-1 induces sterile inflammation in the ischemic brain.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 739
Custom Conjugations