CD11b antibody [M1/70] (FITC)

Cat. No. GTX80051

Host	Rat
Clonality	Monoclonal
Clone Name	M1/70
Isotype	IgG2b
Application	FACS
Reactivity	Human, Mouse, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package	100 μg ($339)

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View product citations for antibody GTX80051 on CiteAb

PRODUCT

Summary

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

B10 mouse spleen cells enriched for T cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin alpha M , CD11b/CD18 , CR3 , CR3A , Cd11b , F730045J24Rik , Ly-40 , MAC1 , Mac-1 , Mac-1a

Database

Gene ID: 16409 Itgam

Research Area

DATA IMAGES

GTX80051 FACS Image

FACS analysis of C57Bl/6 bone marrow cells using GTX80051 CD11b antibody [M1/70] (FITC).
Solid line : Primary antibody
Dashed line : FITC rat IgG2b isotype control
Antibody amount : 0.5 μg