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CD11b antibody [M1/70]

Cat. No. GTX00608

Reference 11

Host

 Rat

Clonality

 Monoclonal

Clone Name

 M1/70

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rabbit, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package
100 μg ($399)
See all CD11b products
PRODUCT

Summary

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

B10 mouse spleen cells enriched for T cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin alpha M , CD11b/CD18 , CR3 , CR3A , Cd11b , F730045J24Rik , Ly-40 , MAC1 , Mac-1 , Mac-1a

Database

Research Area

REFERENCE

Clone Reference

2016; Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion
Application : IP
Reactivity : Mouse
2012; Distinct roles for talin-1 and kindlin-3 in LFA-1 extension and affinity regulation
Application : Neutralizing/Inhibition
Reactivity : Mouse
2010; Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage
Reactivity : Rhesus Monkey
2009; Inhibition of vascular endothelial growth factor reduces angiogenesis and modulates immune cell infiltration of orthotopic breast cancer xenografts
Application : IHC-Fr
Reactivity : Mouse
2008; Early rise in inflammation and microcirculatory disorder determine the development of autoimmune pancreatitis in the MRL/Mp-mouse
Application : IHC-P
Reactivity : Mouse
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REVIEW

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Clone Reference
2016; Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion
Application : IP
Reactivity : Mouse
2012; Distinct roles for talin-1 and kindlin-3 in LFA-1 extension and affinity regulation
Application : Neutralizing/Inhibition
Reactivity : Mouse
2010; Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage
Reactivity : Rhesus Monkey
2009; Inhibition of vascular endothelial growth factor reduces angiogenesis and modulates immune cell infiltration of orthotopic breast cancer xenografts
Application : IHC-Fr
Reactivity : Mouse
2008; Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms
Application : ICC/IF
Reactivity : Human
2008; Regulation of immune response and inflammatory reactions against viral infection by VCAM-1
Application : IHC-Fr
Reactivity : Mouse
2008; Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg
Application : Neutralizing/Inhibition
Reactivity : Mouse
2008; Early rise in inflammation and microcirculatory disorder determine the development of autoimmune pancreatitis in the MRL/Mp-mouse
Application : IHC-P
Reactivity : Mouse
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
Application : Depletion
Reactivity : Mouse
1998; A mAb to the beta2-leukocyte integrin Mac-1 (CD11b/CD18) reduces intimal thickening after angioplasty or stent implantation in rabbits
Reactivity : Rabbit
1995; Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies
Application : IHC-P, IHC-Fr
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399