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CD11b antibody [OX-42] (Biotin)

Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827
Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827

Cat. No. GTX53827

Reference 11

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OX-42

Isotype

 IgG2a

Application

 IHC-Fr, FACS, IHC

Reactivity

 Rat
Package
100 μg ($399)
See all CD11b products

APPLICATION

Application Note

This antibody has been used in Immunohistochemistry (Frozen sections), Flow Cytometry / FACS. Recomended dilutions - FACS: Use neat - 1/10, or 10μl of the suggested working dilution to label 106 cells in 100μl. IHC-Fr: Use at an assay dependent dilution. Note: samples need to be PLP (periodate-lysine-paraformaldehyde) fixed. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Rat CD11b/c

PROPERTIES

Form

Liquid

Buffer

PBS, BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat peritoneal macrophages

Purification

Protein G affinity purified

Conjugation

Biotin

RRID

AB_625703

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Integrin Subunit Alpha M , Cd11B , Itgam

Background

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.GTX53827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.

Research Area

DATA IMAGES

Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827

GTX53827 FACS Image

Staining of rat peritoneal macrophages with Mouse anti Rat CD11b (GTX76060)

Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827

GTX53827 FACS Image

FACS analysis of rat peritoneal macrophages using GTX53827.

REFERENCE

Clone Reference

Li S et al. Mol Med 2022; 28 (1):133 Bone marrow mesenchymal stem cell-derived exosomes shuttling miR-150-5p alleviates mechanical allodynia in rats by targeting NOTCH2 in microglia.
Lee H.P. et al. ournal of Functional Foods 2021; 86:104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Aminzadeh A et al. Cell J 2020; 22 (Suppl 1):38-48 Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
Truzzi F et al. Int J Mol Sci 2020; 22 (1) Pro-Inflammatory Effect of Gliadins and Glutenins Extracted from Different Wheat Cultivars on an In Vitro 3D Intestinal Epithelium Model.
Bai N et al. J Neuroinflammation 2020; 17 (1):45 G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense.
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Application Reference
Nakajima H et al. J Neurotrauma 2012; 29 (8):1614-25 Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury.
Application : IHC
Reactivity : Rat
Clone Reference
Li S et al. Mol Med 2022; 28 (1):133 Bone marrow mesenchymal stem cell-derived exosomes shuttling miR-150-5p alleviates mechanical allodynia in rats by targeting NOTCH2 in microglia.
Lee H.P. et al. ournal of Functional Foods 2021; 86:104712 Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling.
Aminzadeh A et al. Cell J 2020; 22 (Suppl 1):38-48 Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
Truzzi F et al. Int J Mol Sci 2020; 22 (1) Pro-Inflammatory Effect of Gliadins and Glutenins Extracted from Different Wheat Cultivars on an In Vitro 3D Intestinal Epithelium Model.
Bai N et al. J Neuroinflammation 2020; 17 (1):45 G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense.
Lin CH et al. Cell Mol Neurobiol 2019; 39 (5):591-604 Microglia-Derived Adiposomes are Potential Targets for the Treatment of Ischemic Stroke.
Bonfante R et al. Neuroscience 2018; 391:120-130 The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints.
Lin CH et al. PLoS One 2016; 11 (1):e0146692 Molecular Mechanisms Responsible for Neuron-Derived Conditioned Medium (NCM)-Mediated Protection of Ischemic Brain.
Takahashi K et al. Eur J Pain 2011; 15 (5):467.e1-14 IL-1beta in the trigeminal subnucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury.
Zhao Z et al. Glycoconj J 2009; 26 (2):173-88 Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399