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CD11b antibody [OX-42] (Biotin)

Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827
Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827

Cat No. GTX53827

Host

Mouse

Clonality

Monoclonal

Clone Name

OX-42

Isotype

IgG2a

Application

IHC-Fr, FACS, IHC

Reactivity

Rat
Package
100 μg ($319)

APPLICATION

Application Note

This antibody has been used in Immunohistochemistry (Frozen sections), Flow Cytometry / FACS. Recomended dilutions - FACS: Use neat - 1/10, or 10μl of the suggested working dilution to label 106 cells in 100μl. IHC-Fr: Use at an assay dependent dilution. Note: samples need to be PLP (periodate-lysine-paraformaldehyde) fixed. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Rat CD11b/c

PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline containing 0.02% sodium azide and BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat peritoneal macrophages

Purification

Protein G affinity purified

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Integrin Subunit Alpha M , Cd11B , Itgam

Background

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.GTX53827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.

Research Area

DATA IMAGES

Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827

GTX53827 FACS Image

Staining of rat peritoneal macrophages with Mouse anti Rat CD11b (GTX76060)

Anti-CD11b antibody [OX-42] (Biotin) used in Flow cytometry (FACS). GTX53827

GTX53827 FACS Image

FACS analysis of rat peritoneal macrophages using GTX53827.

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319