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CD11b antibody

Anti-CD11b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32495
Anti-CD11b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32495
Anti-CD11b antibody used in Western Blot (WB). GTX32495
Anti-CD11b antibody used in IHC (Paraffin sections) (IHC-P). GTX32495

Cat No. GTX32495

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human ITGAM (NP_001139280.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit alpha M , CD11B , CR3A , MAC-1 , MAC1A , MO1A , SLEB6

Cellular Localization

Cell membrane

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD11b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32495

GTX32495 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX32495 CD11b antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD11b antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32495

GTX32495 ICC/IF Image

ICC/IF analysis of Raw264.7 cells using GTX32495 CD11b antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD11b antibody used in Western Blot (WB). GTX32495

GTX32495 WB Image

WB analysis of various sample lysates using GTX32495 CD11b antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-CD11b antibody used in IHC (Paraffin sections) (IHC-P). GTX32495

GTX32495 IHC-P Image

IHC-P analysis of rat bone marrow tissue using GTX32495 CD11b antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329