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CD11b/c equivalent antibody [OX 42] (FITC)

Anti-CD11b/c equivalent antibody [OX 42] (FITC) used in Flow cytometry (FACS). GTX21212

Cat. No. GTX21212

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OX 42

Isotype

 IgG2a

Application

 IHC-P, IHC-Fr, FACS

Reactivity

 Rat
Package
100 μg ($399)
See all CD11b products

APPLICATION

Application Note

FACS: Use at a dilution of 1/1 - 1/20 (10μl/10^6cells). IHC-P: Use at a dilution of 1/1 - 1/20. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Recognises a common epitope shared by CD11b/c and precipitates three polypeptides of Mw160kD, 103kD and 95kD; a fainter band may also be seen at 133kD. It reacts with all macrophages in the peritoneal cavity, activated macrophages and Kupffer cells, but with only 35% of alveolar macrophages. It also binds to all granulocytes and to dendritic cells, cells with microglial morphology in brain, sparse thymic cortical cells and epidermal Langerhans cells. It inhibits complement mediated rosettes.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat macrophages

Purification

IgG fraction

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_384624

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Integrin Subunit Alpha M , Cd11B , Itgam

Background

The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b/c equivalent antigen.

Research Area

DATA IMAGES

Anti-CD11b/c equivalent antibody [OX 42] (FITC) used in Flow cytometry (FACS). GTX21212

GTX21212 FACS Image

Staining of rat peritoneal macrophages using CD11b: FITC antibody (GTX21212) and FITC mouse IgG2a isotype control

REFERENCE

Application Reference

Nakajima H et al. J Neurotrauma 2012; 29 (8) : 1614-25 Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury.
Application : IHC-Fr
Reactivity : Rat

Clone Reference

Tang HY et al. J Physiol Sci 2020; 70 (1):45 Acupuncture attenuates the development of diabetic peripheral neuralgia by regulating P2X4 expression and inflammation in rat spinal microglia.
Bonfante R et al. Neuroscience 2018; 391:120-130 The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399