GeneTex
United States (US)

CD11c antibody [ITGAX/1242]

GTX34455_20170712_IHC-P_18121410_507.jpg

Cat No. GTX34455

Host Mouse
Clonality Monoclonal
Clone Name ITGAX/1242
Isotype IgG2b
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:.

Calculated MW

128 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human ITGAX protein fragment (aa 637-827) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Alpha X,Cd11C,Sleb6,Itgax

Background

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES
GTX34455_20170712_IHC-P_18121410_507.jpg

GTX34455 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD11c Monoclonal Antibody (ITGAX/1242) at 1ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of dendritic cells.

Package List Price ($)
$ 329