Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1/50-1/100 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells 100μl whole blood.
Neutralizing/Inhibition
Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachance et al. 1998) but not hepatitis C virus (Koutsoudakis et al. 2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmun et al. 1992).
|
Not tested in other applications.
Calculated MW
Product Note
We would not recommend using this antibody for WB. For human WB, please using GTX102788.
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human AML cells.
Purification
Protein A purified
From tissue culture supernatant
Endotoxin
< 0.01 EU/μg (determined by the LAL assay)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
alanyl aminopeptidase, membrane , APN , CD13 , GP150 , LAP1 , P150 , PEPN
Cellular Localization
Cell membrane
Background
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]
Database
Research Area
