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CD13 antibody

Anti-CD13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64331
Anti-CD13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64331
Anti-CD13 antibody used in Western Blot (WB). GTX64331
Anti-CD13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64331

Cat. No. GTX64331

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 668-967 of human ANPEP (NP_001141.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alanyl aminopeptidase, membrane , APN , CD13 , GP150 , LAP1 , P150 , PEPN

Cellular Localization

Cell membrane

Background

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64331

GTX64331 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64331 CD13 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64331

GTX64331 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64331 CD13 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD13 antibody used in Western Blot (WB). GTX64331

GTX64331 WB Image

WB analysis of various sample lysates using GTX64331 CD13 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64331

GTX64331 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64331 CD13 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399