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CD13 antibody

Anti-CD13 antibody used in Western Blot (WB). GTX66718
Anti-CD13 antibody used in IHC (Paraffin sections) (IHC-P). GTX66718

Cat No. GTX66718

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 880-930 of Human CD13.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

alanyl aminopeptidase, membrane , APN , CD13 , GP150 , LAP1 , P150 , PEPN

Cellular Localization

Cell membrane

Background

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD13 antibody used in Western Blot (WB). GTX66718

GTX66718 WB Image

WB analysis of various samples using GTX66718 CD13 antibody.
Lane1 : Mouse liver tissue lysate
Lane2 : Rat liver tissue lysate
Dilution : 1:500

Anti-CD13 antibody used in IHC (Paraffin sections) (IHC-P). GTX66718

GTX66718 IHC-P Image

IHC-P analysis of human kidney tissue using GTX66718 CD13 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf