Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD130 antibody (GTX109048) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD130(GTX109048) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CD130 antibody detects CD130 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CD130 stained by CD130 antibody (GTX109048) diluted at 1:7500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min