GeneTex
United States (US)

CD133 antibody [3F10]

Anti-CD133 antibody [3F10] used in ELISA (ELISA). GTX60471
Anti-CD133 antibody [3F10] used in IHC (Paraffin sections) (IHC-P). GTX60471
Anti-CD133 antibody [3F10] used in Flow cytometry (FACS). GTX60471

Cat No. GTX60471

Host Mouse
Clonality Monoclonal
Clone Name 3F10
Isotype IgG1
Application IHC-P, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide of human CD133.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prominin 1,Ac133,Cd133,Cord12,Mcdr2,Mstp061,Proml1,Rp41,Stgd4,Prom1

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES
Anti-CD133 antibody [3F10] used in ELISA (ELISA). GTX60471

GTX60471 ELISA Image

ELISA analysis of antigen using GTX60471 CD133 antibody [3F10].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-CD133 antibody [3F10] used in IHC (Paraffin sections) (IHC-P). GTX60471

GTX60471 IHC-P Image

IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]

Anti-CD133 antibody [3F10] used in Flow cytometry (FACS). GTX60471

GTX60471 FACS Image

FACS analysis of HeLa cells using GTX60471 CD133 antibody [3F10].
Blue : CD133
Red : negative control

REFERENCE
Package List Price ($)
$ 329