GeneTex

  • Country / Location Selection

United States (US)

CD133 antibody [3F10]

Anti-CD133 antibody [3F10] used in IHC (Paraffin sections) (IHC-P). GTX60471
Anti-CD133 antibody [3F10] used in ELISA (ELISA). GTX60471
Anti-CD133 antibody [3F10] used in Flow cytometry (FACS). GTX60471

Cat. No. GTX60471

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F10

Isotype

 IgG1

Application

 IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($419)
See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide of human CD133.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane,Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody [3F10] used in IHC (Paraffin sections) (IHC-P). GTX60471

GTX60471 IHC-P Image

IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]

Anti-CD133 antibody [3F10] used in ELISA (ELISA). GTX60471

GTX60471 ELISA Image

ELISA analysis of antigen using GTX60471 CD133 antibody [3F10].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-CD133 antibody [3F10] used in Flow cytometry (FACS). GTX60471

GTX60471 FACS Image

FACS analysis of HeLa cells using GTX60471 CD133 antibody [3F10].
Blue : CD133
Red : negative control

REFERENCE

Application Reference

Kim J et al. J Gastrointest Oncol 2021; 12 (4) : 1326-1337 Slug and CD133 expression are associated with peritoneal carcinomatosis and survival in gastric cancer.
Application : IHC-P
Reactivity : Human
Luna ECM et al. Asian Pac J Cancer Prev 2020; 21 (9) : 2501-2506 CD133 Role in Oral Carcinogenesis.
Luna, Ealber Carvalho Macedo et al. 0 2015; Express?o imuno-histoqu?mica de cd133 em displasias epiteliais orais e carcinomas epidermoides orais.
Submit a Reference

REVIEW

There are currently no reviews for CD133 antibody [3F10] (GTX60471). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 419