APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of Human CD133. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910041
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4
Cellular Localization
Apical cell membrane,Endoplasmic reticulum
Background
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/templates/default/js/lightbox/images/loading.gif "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
|
GTX636673 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX636673 IHC-P Image
CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CD133 stained by CD133 antibody [HL1271] (GTX636673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX636673 IHC-P Image
CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.CD133 stained by CD133 antibody [HL1271] (GTX636673) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX636673 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for CD133 antibody [HL1271] (GTX636673). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD133 antibody [HL1271] (GTX636673). Be the first to share your experience with this product.
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_44599_20220225_WB_22030718_281.jpg "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_M_22122821_353.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_R_22122821_743.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/small/GTX636673/GTX636673_T-44536_20220121_WB_B_22012500_673.jpg "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20220225_WB_22030718_281.jpg "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_M_22122821_353.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20221230_IHC-P_multiple_R_22122821_743.webp "Anti-CD133 antibody [HL1271] used in IHC (Paraffin sections) (IHC-P). GTX636673")
![Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673](/upload/website/prouct_img/normal/GTX636673/GTX636673_T-44536_20220121_WB_B_22012500_673.jpg "Anti-CD133 antibody [HL1271] used in Western Blot (WB). GTX636673")