GeneTex
United States (US)

CD133 antibody [N1C1]

Overexpression
GTX102109_39549_WB_B_19011506_100.jpg
GTX102109_WB_19011506_990.jpg

Cat No. GTX102109

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HCT116 , human CD133-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prominin 1,Ac133,Cd133,Cord12,Mcdr2,Mstp061,Proml1,Rp41,Stgd4,Prom1

Cellular Localization

Apical cell membrane; Multi-pass membrane protein , Cell projection , microvillus membrane; Multi-pass membrane protein

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102109_39549_WB_B_19011506_100.jpg

GTX102109 WB Image

Overexpression

CD133 antibody [N1C1] detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [N1C1] (GTX102109) dilution: 1:5000

GTX102109_WB_19011506_990.jpg

GTX102109 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116 7.5% SDS PAGE
GTX102109 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169