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CD133 antibody [N1C1]

Overexpression
Anti-CD133 antibody [N1C1] used in Western Blot (WB). GTX102109
Anti-CD133 antibody [N1C1] used in Western Blot (WB). GTX102109
Anti-CD133 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102109

Cat. No. GTX102109

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

3xFlag-human CD133-transfected 293T cells

Product Note

Based on internal testing result, this antibody may cross react with EphA5, EphA6, and EphA7.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD133. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240579

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prominin 1 , AC133 , CD133 , CORD12 , MCDR2 , MSTP061 , PROML1 , RP41 , STGD4

Cellular Localization

Apical cell membrane,Endoplasmic reticulum

Background

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CD133 antibody [N1C1] used in Western Blot (WB). GTX102109

GTX102109 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 29760280

Anti-CD133 antibody [N1C1] used in Western Blot (WB). GTX102109

GTX102109 WB Image

Overexpression

CD133 antibody [N1C1] detects CD133 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human CD133-transfected 293T cells
7.5 % SDS-PAGE
CD133 antibody [N1C1] (GTX102109) dilution: 1:5000

Anti-CD133 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102109

GTX102109 IHC-P Image

CD133 antibody [N1C1] detects CD133 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CD133 stained by CD133 antibody [N1C1] (GTX102109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

BY Wang et al. Am J Cancer Res 2023; 13 (10) : 4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Huang TH et al. J Cell Mol Med 2019; 23 (12) : 8184-8195 In silico identification of thiostrepton as an inhibitor of cancer stem cell growth and an enhancer for chemotherapy in non-small-cell lung cancer.
Application : WB
Reactivity : Human
Hong KJ et al. J Cell Physiol 2014; 229 (2) : 191-201 RECK inhibits stemness gene expression and tumorigenicity of gastric cancer cells by suppressing ADAM-mediated Notch1 activation.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169