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CD134 antibody [OX-40] (FITC)

Anti-CD134 antibody [OX-40] (FITC) used in Flow cytometry (FACS). GTX76450

Cat. No. GTX76450

Reference 13

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OX-40

Isotype

 IgG2b

Application

 FACS

Reactivity

 Rat
Package
100 μg ($419)
See all CD134 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Phytohemagglutinin (PHA) activated Rat Lymph Node Cells.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_379303

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor superfamily member 4 , Ox40 , Txgp1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

membrane-bound receptor, binds adaptor proteins Traf2 and Traf5; member of the tumor necrosis factor (TNF)-receptor superfamily [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-CD134 antibody [OX-40] (FITC) used in Flow cytometry (FACS). GTX76450

GTX76450 FACS Image

FACS analysis of stimulated rat spleen cells using GTX76450 CD134 antibody [OX-40] (FITC).

REFERENCE

Clone Reference

Snelgrove RJ et al. J Infect Dis 2012; 205 (6):975-83 OX40 ligand fusion protein delivered simultaneously with the BCG vaccine provides superior protection against murine Mycobacterium tuberculosis infection.
Volovitz I et al. J Autoimmun 2010; 35 (2):135-44 T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
Albillos A et al. Gut 2010; 59 (7):943-52 The biological response modifier AM3 attenuates the inflammatory cell response and hepatic fibrosis in rats with biliary cirrhosis.
Thaunat O et al. PLoS One 2010; 5 (6):e11398 Immune responses elicited in tertiary lymphoid tissues display distinctive features.
Giorgini A et al. J Leukoc Biol 2007; 82 (5):1053-61 Blockade of chronic graft-versus-host disease by alloantigen-induced CD4+CD25+Foxp3+ regulatory T cells in nonlymphopenic hosts.
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Clone Reference
Snelgrove RJ et al. J Infect Dis 2012; 205 (6):975-83 OX40 ligand fusion protein delivered simultaneously with the BCG vaccine provides superior protection against murine Mycobacterium tuberculosis infection.
Volovitz I et al. J Autoimmun 2010; 35 (2):135-44 T cell vaccination induces the elimination of EAE effector T cells: analysis using GFP-transduced, encephalitogenic T cells.
Albillos A et al. Gut 2010; 59 (7):943-52 The biological response modifier AM3 attenuates the inflammatory cell response and hepatic fibrosis in rats with biliary cirrhosis.
Thaunat O et al. PLoS One 2010; 5 (6):e11398 Immune responses elicited in tertiary lymphoid tissues display distinctive features.
Giorgini A et al. J Leukoc Biol 2007; 82 (5):1053-61 Blockade of chronic graft-versus-host disease by alloantigen-induced CD4+CD25+Foxp3+ regulatory T cells in nonlymphopenic hosts.
Cousins L et al. Gastroenterology 2006; 131 (5):1475-85 Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene.
Stephens LA et al. Int Immunol 2004; 16 (2):365-75 Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
Humphreys IR et al. J Exp Med 2003; 198 (8):1237-42 A critical role for OX40 in T cell-mediated immunopathology during lung viral infection.
Lavelle EC et al. J Immunol 2003; 171 (5):2384-92 Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation.
Walker LS et al. J Exp Med 1999; 190 (8):1115-22 Compromised OX40 function in CD28-deficient mice is linked with failure to develop CXC chemokine receptor 5-positive CD4 cells and germinal centers.
Richardson PR et al. Br J Ophthalmol 1996; 80 (12):1092-8 Immunocytochemical study of retinal diode laser photocoagulation in the rat.
Mallett S et al. EMBO J 1990; 9 (4):1063-8 Characterization of the MRC OX40 antigen of activated CD4 positive T lymphocytes--a molecule related to nerve growth factor receptor.
Paterson DJ et al. Mol Immunol 1987; 24 (12):1281-90 Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419