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CD134 antibody [OX86] (PE)

Anti-CD134 antibody [OX86] (PE) used in Flow cytometry (FACS). GTX74996

Cat. No. GTX74996

Reference 11

Host

 Rat

Clonality

 Monoclonal

Clone Name

 OX86

Isotype

 IgG1

Application

 IHC-Fr, FACS, Blocking

Reactivity

 Mouse
Package
50 test ($399)
See all CD134 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS Neat
Blocking Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 5% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

CHO cells transfected with murine CD134

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

RRID

AB_378322

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor receptor superfamily, member 4 , ACT35 , CD134 , Ly-70 , Ox40 , TXGP1L , Txgp1

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD134 antibody [OX86] (PE) used in Flow cytometry (FACS). GTX74996

GTX74996 FACS Image

FACS analysis of Con A activated mouse spleen cells using GTX74996 CD134 antibody [OX86] (PE).

REFERENCE

Clone Reference

Wythe SE et al. J Immunol 2012; 188 (4):1647-55 OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease.
Maenz M et al. Mol Vis 2011; 17:420-9 A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
Application : FACS
Thaunat O et al. PLoS One 2010; 5 (6):e11398 Immune responses elicited in tertiary lymphoid tissues display distinctive features.
Application : FACS
Zaini J et al. J Clin Invest 2007; 117 (11):522511 OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
Application : IHC-Fr
Andarini S et al. Cancer Res 2004; 64 (9):504584 Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.
Application : IHC-Fr
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REVIEW

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Clone Reference
Wythe SE et al. J Immunol 2012; 188 (4):1647-55 OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease.
Maenz M et al. Mol Vis 2011; 17:420-9 A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
Application : FACS
Thaunat O et al. PLoS One 2010; 5 (6):e11398 Immune responses elicited in tertiary lymphoid tissues display distinctive features.
Application : FACS
Zaini J et al. J Clin Invest 2007; 117 (11):522511 OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
Application : IHC-Fr
Lee SJ et al. J Immunol 2004; 173 (5):402833 4-1BB and OX40 dual costimulation synergistically stimulate primary specific CD8 T cells for robust effector function.
Application : Blocking
Andarini S et al. Cancer Res 2004; 64 (9):504584 Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.
Application : IHC-Fr
Lavelle EC et al. J Immunol 2003; 171 (5):2384-92 Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation.
Application : FACS
McHugh RS et al. Immunity 2002; 16 (2):311-23 CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.
Malmstrom V et al. J Immunol 2001; 166 (11):6972-81 CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.
Application : FACS
Takeda K et al. J Immunol 2000; 164 (4):1741-5 CD27-mediated activation of murine NK cells.
Higgins LM et al. J Immunol 1999; 162 (1):486-93 Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
Application : IHC-Fr
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399