GeneTex
United States (US)

CD137 antibody

Cat No. GTX10459

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 μg/ml
ICC/IF 5-15 μg/ml
FACS 2.5 μg/10⁶ cells
ELISA 0.2-0.8 μg/ml
Not tested in other applications.

Calculated MW

28 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu24Gln186

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Receptor Superfamily Member 9,4-1Bb,Cd137,Cdw137,Ila,Tnfrsf9

Background

CD137 exists on the cell surface as a monomer with a molecular mass of 30 kDa and as a dimer of 55 kDa. Human and mouse CD137 share 60% amino acid identity. CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival.

Database

Research Area

Package List Price ($)
$ 319