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CD14 antibody, Internal

Anti-CD14 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89394
Anti-CD14 antibody, Internal used in Western Blot (WB). GTX89394

Cat. No. GTX89394

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($419)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89394-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 molecule

Cellular Localization

Cell membrane , Secreted , Golgi apparatus

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89394

GTX89394 IHC-P Image

IHC-P analysis of human spleen using GTX89394 CD14 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml

Anti-CD14 antibody, Internal used in Western Blot (WB). GTX89394

GTX89394 WB Image

WB analysis of peripheral blood lymphocyte lysate with (B) and without (A) blocking with the immunising peptide using GTX89394 CD14 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419